S1PR1 monoclonal antibody, clone 1E3H5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: S1PR1-hIgGFc transfected HEK293 cell lysates with S1PR1 monoclonal antibody, clone 1E3H5 (Cat # MAB21369).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with S1PR1 monoclonal antibody, clone 1E3H5 (Cat # MAB21369).
Flow Cytometry
Flow cytometric analysis of HL-60 cells with S1PR1 monoclonal antibody, clone 1E3H5 (Cat # MAB21369) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human S1PR1.
Immunogen
Recombinant protein corresponding to human S1PR1.
Host
Mouse
Theoretical MW (kDa)
42.8
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: S1PR1-hIgGFc transfected HEK293 cell lysates with S1PR1 monoclonal antibody, clone 1E3H5 (Cat # MAB21369).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with S1PR1 monoclonal antibody, clone 1E3H5 (Cat # MAB21369).Flow Cytometry
Flow cytometric analysis of HL-60 cells with S1PR1 monoclonal antibody, clone 1E3H5 (Cat # MAB21369) (Green). Red: Negative Control. -
Gene Info — S1PR1
Entrez GeneID
1901Protein Accession#
P21453Gene Name
S1PR1
Gene Alias
CHEDG1, D1S3362, ECGF1, EDG-1, EDG1, FLJ58121, S1P1
Gene Description
sphingosine-1-phosphate receptor 1
Omim ID
601974Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq
Other Designations
G protein-coupled sphingolipid receptor|OTTHUMP00000012525|endothelial differentiation, sphingolipid G-protein-coupled receptor, 1|sphingosine 1-phosphate receptor EDG1
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Interactome
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Pathway
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Disease
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