PTPRJ monoclonal antibody, clone 1D2C11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: PTPRJ-hIgGFc transfected HEK293 cell lysates with PTPRJ monoclonal antibody, clone 1D2C11 (Cat # MAB21367).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with PTPRJ monoclonal antibody, clone 1D2C11 (Cat # MAB21367).
Flow Cytometry
Flow cytometric analysis of HL-60 cells with PTPRJ monoclonal antibody, clone 1D2C11 (Cat # MAB21367) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human PTPRJ.
Immunogen
Recombinant protein corresponding to amino acids 36-210 of human PTPRJ.
Host
Mouse
Theoretical MW (kDa)
146
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: PTPRJ-hIgGFc transfected HEK293 cell lysates with PTPRJ monoclonal antibody, clone 1D2C11 (Cat # MAB21367).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with PTPRJ monoclonal antibody, clone 1D2C11 (Cat # MAB21367).Flow Cytometry
Flow cytometric analysis of HL-60 cells with PTPRJ monoclonal antibody, clone 1D2C11 (Cat # MAB21367) (Green). Red: Negative Control. -
Gene Info — PTPRJ
Entrez GeneID
5795Protein Accession#
Q12913Gene Name
PTPRJ
Gene Alias
CD148, DEP1, HPTPeta, R-PTP-ETA, SCC1
Gene Description
protein tyrosine phosphatase, receptor type, J
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD148 antigen|human density enhanced phosphatase-1|protein tyrosine phosphatase, receptor type, J polypeptide|receptor-type tyrosine-protein phosphatase eta|susceptibility to colon cancer 1, mouse, homolog of
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Interactome
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Pathway
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Disease
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