TEK monoclonal antibody, clone 3F5B9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: TEK-hIgGFc transfected HEK293 cell lysates with TEK monoclonal antibody, clone 3F5B9 (Cat # MAB21352).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with TEK monoclonal antibody, clone 3F5B9 (Cat # MAB21352).
Flow Cytometry
Flow cytometric analysis of K562 cells with TEK monoclonal antibody, clone 3F5B9 (Cat # MAB21352) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human TEK.
Immunogen
Recombinant protein corresponding to amino acids 571-748 of human TEK.
Host
Mouse
Theoretical MW (kDa)
125.8
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: TEK-hIgGFc transfected HEK293 cell lysates with TEK monoclonal antibody, clone 3F5B9 (Cat # MAB21352).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with TEK monoclonal antibody, clone 3F5B9 (Cat # MAB21352).Flow Cytometry
Flow cytometric analysis of K562 cells with TEK monoclonal antibody, clone 3F5B9 (Cat # MAB21352) (Green). Red: Negative Control. -
Gene Info — TEK
Entrez GeneID
7010Protein Accession#
Q02763Gene Name
TEK
Gene Alias
CD202B, TIE-2, TIE2, VMCM, VMCM1
Gene Description
TEK tyrosine kinase, endothelial
Gene Ontology
HyperlinkGene Summary
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. [provided by RefSeq
Other Designations
OTTHUMP00000021167|soluble TIE2 variant 1|soluble TIE2 variant 2
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Interactome
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Disease
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