TIGIT monoclonal antibody, clone 4C5B9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: TIGIT-hIgGFc transfected HEK293 cell lysates with TIGIT monoclonal antibody, clone 4C5B9 (Cat # MAB21338).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with TIGIT monoclonal antibody, clone 4C5B9 (Cat # MAB21338).
Flow Cytometry
Flow cytometric analysis of K562 cells with TIGIT monoclonal antibody, clone 4C5B9 (Cat # MAB21338) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human TIGIT.
Immunogen
Recombinant protein corresponding to amino acids 22-141 of human TIGIT.
Host
Mouse
Theoretical MW (kDa)
26.3
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: TIGIT-hIgGFc transfected HEK293 cell lysates with TIGIT monoclonal antibody, clone 4C5B9 (Cat # MAB21338).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with TIGIT monoclonal antibody, clone 4C5B9 (Cat # MAB21338).Flow Cytometry
Flow cytometric analysis of K562 cells with TIGIT monoclonal antibody, clone 4C5B9 (Cat # MAB21338) (Green). Red: Negative Control. -
Gene Info — TIGIT
Entrez GeneID
201633Protein Accession#
Q495A1Gene Name
TIGIT
Gene Alias
DKFZp667A205, FLJ39873, VSIG9, VSTM3
Gene Description
T cell immunoreceptor with Ig and ITIM domains
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses
Other Designations
V-set and immunoglobulin domain containing 9|V-set and transmembrane domain containing 3
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