CPV-NS1 monoclonal antibody, clone 2B5B7
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CPV-NS1.
Immunogen
Recombinant protein corresponding to amino acids 544-668 of human CPV-NS1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunofluorescence (1:200-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: CPV-NS1-hIgGFc transfected HEK293 cell lysates with CPV-NS1 monoclonal antibody, clone 2B5B7 (Cat # MAB21317).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence
Immunofluorescent staining of HeLa cells with CPV-NS1 monoclonal antibody, clone 2B5B7 (Cat # MAB21317) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis with CPV-NS1 monoclonal antibody, clone 2B5B7 (Cat # MAB21317).Flow Cytometry
Flow cytometric analysis of HeLa cells with CPV-NS1 monoclonal antibody, clone 2B5B7 (Cat # MAB21317) (Green). Red: Negative Control.
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