PGRMC1 monoclonal antibody, clone 6F9A1
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human PGRMC1.
Immunogen
Recombinant protein corresponding to full length human PGRMC1.
Host
Mouse
Theoretical MW (kDa)
21.7
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunofluorescence (1:200-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: PGRMC1-hIgGFc transfected HEK293 cell lysates with PGRMC1 monoclonal antibody, clone 6F9A1 (Cat # MAB21314).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with PGRMC1 monoclonal antibody, clone 6F9A1 (Cat # MAB21314).Immunofluorescence
Immunofluorescent staining of MCF-7 cells with PGRMC1 monoclonal antibody, clone 6F9A1 (Cat # MAB21314) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA analysis with PGRMC1 monoclonal antibody, clone 6F9A1 (Cat # MAB21314).Flow Cytometry
Flow cytometric analysis of A549 cells with PGRMC1 monoclonal antibody, clone 6F9A1 (Cat # MAB21314) (Green). Red: Negative Control. -
Gene Info — PGRMC1
Entrez GeneID
10857Protein Accession#
O00264Gene Name
PGRMC1
Gene Alias
HPR6.6, MPR
Gene Description
progesterone receptor membrane component 1
Omim ID
300435Gene Ontology
HyperlinkGene Summary
Progesterone binding protein is a putative steroid membrane receptor. The protein is expressed predominantly in the liver and kidney. [provided by RefSeq
Other Designations
OTTHUMP00000023897|progesterone binding protein
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Interactome
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Disease
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