MKI67 monoclonal antibody, clone MKI67/2462
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human tonsil.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skin.
Immunofluorescence
Immunofluorescent staining of HeLa cells labeled with CF488 (Green) and Phalloidin (Red) is used to label the membranes.
Flow Cytometry
Flow cytometric analysis of HeLa cells. Goat anti-mouse IgG-CF488 (Blue) ; Isotype Control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial human MKI67.
Immunogen
Recombinant protein corresponding to amino acids 2293-2478 of human MKI67.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2b, kappa
Recommend Usage
Flow Cytometry (1-2 ug/million cells) (HeLa cells trypsinised 0.25% for 2-4minutes, 2-4% PFA fixed)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 1 mg/mL PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human tonsil.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skin.Immunofluorescence
Immunofluorescent staining of HeLa cells labeled with CF488 (Green) and Phalloidin (Red) is used to label the membranes.Flow Cytometry
Flow cytometric analysis of HeLa cells. Goat anti-mouse IgG-CF488 (Blue) ; Isotype Control (Red). -
Gene Info — MKI67
Entrez GeneID
4288Protein Accession#
P46013Gene Name
MKI67
Gene Alias
KIA, Ki-67
Gene Description
antigen identified by monoclonal antibody Ki-67
Omim ID
176741Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq
Other Designations
OTTHUMP00000020739|proliferation-related Ki-67 antigen
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Interactome
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Disease
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