MKI67 monoclonal antibody, clone MKI67/2461
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human tonsil.
Immunofluorescence
Immunofluorescent staining of HeLa cells labeled with CF488 (Green) ; WGA (Red) is used to label the membrane.
Flow Cytometry
Flow cytometric analysis of HeLa cells. Goat anti-mouse IgG-CF488 (Blue) ; Isotype Control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial human MKI67.
Immunogen
Recombinant protein corresponding to amino acids 2293-2478 of human MKI67.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (1-2 ug/million cells) (HeLa cells trypsinised 0.25% for 2-4minutes, 2-4% PFA fixed)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% BSA and 0.05% azide).
Storage Instruction
Store at 2 to 8°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human tonsil.Immunofluorescence
Immunofluorescent staining of HeLa cells labeled with CF488 (Green) ; WGA (Red) is used to label the membrane.Flow Cytometry
Flow cytometric analysis of HeLa cells. Goat anti-mouse IgG-CF488 (Blue) ; Isotype Control (Red). -
Gene Info — MKI67
Entrez GeneID
4288Protein Accession#
P46013Gene Name
MKI67
Gene Alias
KIA, Ki-67
Gene Description
antigen identified by monoclonal antibody Ki-67
Omim ID
176741Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq
Other Designations
OTTHUMP00000020739|proliferation-related Ki-67 antigen
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Interactome
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Disease
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