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Last updated: 2022/7/3
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CD274 monoclonal antibody, clone PDL1/2746 

  • Catalog # : MAB21032
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human CD274.
  • Immunogen:
  • Recombinant protein corresponding to around amino acid 39-191 of human CD274. (exact sequence is proprietary)
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 37-50
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • ELISA (For coating)
    Flow Cytometry (1-2 ug/million cells)
    Immunofluorescence (1-2 ug/mL)
    Immunohistochemistry (Formalin-fixed) (1-2 ug/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
    Western Blot (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS.
  • Storage Instruction:
  • Store at -20 to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of (1) HEK293 and (2) HepG2 cell lysates using CD274 monoclonal antibody, clone PDL1/2746.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human Lung SCC using CD274 monoclonal antibody, clone PDL1/2746.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of Jurkat cells labeling CD274 with CD274 monoclonal antibody, clone PDL1/2746.
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of human Jurkat cells using CD274 monoclonal antibody, clone PDL1/2746.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Gene Name:
  • CD274
  • Gene Alias:
  • B7-H,B7H1,MGC142294,MGC142296,PD-L1,PDCD1L1,PDCD1LG1,PDL1
  • Gene Description:
  • CD274 molecule
  • Other Designations:
  • CD274 antigen,OTTHUMP00000021029,programmed cell death 1 ligand 1
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