Mouse monoclonal antibody raised against partial recombinant human CD274.
Immunogen:
Recombinant protein corresponding to around amino acid 39-191 of human CD274. (exact sequence is proprietary)
Host:
Mouse
Theoretical MW (kDa):
37-50
Reactivity:
Human
Form:
Liquid
Purification:
Protein A/G purification
Isotype:
IgG2b, kappa
Recommend Usage:
ELISA (For coating) Flow Cytometry (1-2 ug/million cells) Immunofluorescence (1-2 ug/mL) Immunohistochemistry (Formalin-fixed) (1-2 ug/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In 10 mM PBS.
Storage Instruction:
Store at -20 to -80°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.