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Last updated: 2016/12/4
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Stat5a (phospho Y694) monoclonal antibody, clone 5F6.F1

  • Catalog # : MAB2097
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic phosphopeptide of Stat5a.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding Y694 of mouse Stat5a.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody is specific to mouse Stat5alpha protein phosphorylated at Y694.
  • Form:
  • Liquid
  • Isotype:
  • IgG1, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:20000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (20-40 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using Stat5a (phospho Y694) monoclonal antibody, clone 5F6.F1 (Cat # MAB2097) shows detection of phosphorylated Stat5a (indicated by arrowhead at ~91 KDa) in NK92 cells after 30 min treatment with 1 Ku of IL-2 (lane 2).
    No reactivity is seen for non-phosphorylated Stat5a in untreated cells (lane 1).
    The membrane was probed with the primary antibody at a 1 : 1,000 dilution, overnight at 4°C.
    For detection DyLight™800 conjugated Gt-a-Mouse IgG was used at a 1 : 20,000 dilution for 30 min at room temperature followed by visualization using a VersaDoc™ MP 4000 imaging system (Bio-Rad).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry using Stat5a (phospho Y694) monoclonal antibody, clone 5F6.F1 (Cat # MAB2097) shows detection of phosphorylated Stat5a pY694 in human breast tissue (40X). The antibody was used a dilution to 20 ug/mL . The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
    Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_035618;P42230
  • Gene Name:
  • Stat5a
  • Gene Alias:
  • AA959963,STAT5
  • Gene Description:
  • signal transducer and activator of transcription 5A
  • Other Designations:
  • OTTMUSP00000002045,OTTMUSP00000002046,STAT5a variant delta E18,mammary gland factor STAT5A
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  • Interactome
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