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Last updated: 2017/7/23
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SMT3 monoclonal antibody, clone 4F2.F5.G2

  • Catalog # : MAB2093
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant SMT3.
  • Immunogen:
  • Recombinant protein corresponding to full length Saccharomyces cerevisiae SMT3.
  • Host:
  • Mouse
  • Reactivity:
  • Yeast
  • Form:
  • Liquid
  • Isotype:
  • IgG1, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:20000)
    Western Blot (1:2000)
    Immunohistochemistry (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Immunoblot of SMT3 fusion protein.
    SMT3 monoclonal antibody, clone 4F2.F5.G2 (Cat # MAB2093), generated by immunization with recombinant yeast SMT3, was tested by immunoblot against a SMT3-GFP fusion protein (lane 2).
    While the actual molecular weight of the fusion protein is 39 KDa, the protein migrates as a 49 KDa band (arrowhead).
    No reactivity is seen for lane 1 which contains His-tagged GFP protein.
    The primary antibody was used at a 1:1,000 dilution.
  • Immunohistochemistry
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_010798;Q12306
  • Gene Name:
  • SMT3
  • Gene Alias:
  • -
  • Gene Description:
  • Smt3p
  • Other Designations:
  • Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics
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