Immunoblot of SMT3 fusion protein. SMT3 monoclonal antibody, clone 4F2.F5.G2 (Cat # MAB2093), generated by immunization with recombinant yeast SMT3, was tested by immunoblot against a SMT3-GFP fusion protein (lane 2). While the actual molecular weight of the fusion protein is 39 KDa, the protein migrates as a 49 KDa band (arrowhead). No reactivity is seen for lane 1 which contains His-tagged GFP protein. The primary antibody was used at a 1:1,000 dilution.
Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics