SMT3 monoclonal antibody, clone 4F2.F5.G2
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More Files
- More Functions
Western Blot (Recombinant protein)
Immunoblot of SMT3 fusion protein.
SMT3 monoclonal antibody, clone 4F2.F5.G2 (Cat # MAB2093), generated by immunization with recombinant yeast SMT3, was tested by immunoblot against a SMT3-GFP fusion protein (lane 2).
While the actual molecular weight of the fusion protein is 39 KDa, the protein migrates as a 49 KDa band (arrowhead).
No reactivity is seen for lane 1 which contains His-tagged GFP protein.
The primary antibody was used at a 1:1,000 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant SMT3.
Immunogen
Recombinant protein corresponding to full length Saccharomyces cerevisiae SMT3.
Host
Mouse
Reactivity
Yeast
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:20000)
Western Blot (1:2000)
Immunohistochemistry (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Immunoblot of SMT3 fusion protein.
SMT3 monoclonal antibody, clone 4F2.F5.G2 (Cat # MAB2093), generated by immunization with recombinant yeast SMT3, was tested by immunoblot against a SMT3-GFP fusion protein (lane 2).
While the actual molecular weight of the fusion protein is 39 KDa, the protein migrates as a 49 KDa band (arrowhead).
No reactivity is seen for lane 1 which contains His-tagged GFP protein.
The primary antibody was used at a 1:1,000 dilution.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — SMT3
Entrez GeneID
852122Protein Accession#
NP_010798;Q12306Gene Name
SMT3
Gene Alias
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Gene Description
Smt3p
Gene Ontology
HyperlinkGene Summary
conjugated to lysine residues of target proteins; regulates chromatid cohesion
Other Designations
Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics
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Publication Reference
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SP-RING for SUMO: new functions bloom for a ubiquitin-like protein.
Hochstrasser M.
Cell 2001 Oct; 107(1):5.
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Involvement of PIAS1 in the sumoylation of tumor suppressor p53.
Kahyo T, Nishida T, Yasuda H.
Molecular Cell 2001 Sep; 8(3):713.
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SUMO, ubiquitin's mysterious cousin.
Muller S, Hoege C, Pyrowolakis G, Jentsch S.
Nature Reviews. Molecular Cell Biology 2001 Mar; 2(3):202.
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SP-RING for SUMO: new functions bloom for a ubiquitin-like protein.
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