NEDD9 monoclonal antibody, clone 14A11
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More Files
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Western Blot (Cell lysate)
Western blotting using NEDD9 monoclonal antibody, clone 14A11 (Cat # MAB2091) shows detection of NEDD9 present in MCF-7 cells induced to express NEDD9 by tetracycline removal (right lane).
See Pugacheva et al for details.
Immunofluorescence
Immunofluorescence microscopyusing NEDD9 monoclonal antibody, clone 14A11 (Cat # MAB2091) shows detectionof NEDD9 localized at the centrosome (bright dots) and focal adhesion sites.
The antibody was used at a 1:100 dilution.
Personal Communication. Elena Pugacheva, FoxChase Cancer Center, Philadelphia, PA.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant NEDD9.
Immunogen
Recombinant GST fusion protein corresponding to amino acids 82-398 of human NEDD9.
Host
Mouse
Reactivity
Human
Specificity
This Protein A purified antibody is directed against human HEF1 protein. Reactivity against multiple isoforms is expected. Specificity was determined by partial epitope mapping.
Form
Liquid
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:500)
Immunofluorescence (1:100)
Immunoprecipitation (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blotting using NEDD9 monoclonal antibody, clone 14A11 (Cat # MAB2091) shows detection of NEDD9 present in MCF-7 cells induced to express NEDD9 by tetracycline removal (right lane).
See Pugacheva et al for details.Immunofluorescence
Immunofluorescence microscopyusing NEDD9 monoclonal antibody, clone 14A11 (Cat # MAB2091) shows detectionof NEDD9 localized at the centrosome (bright dots) and focal adhesion sites.
The antibody was used at a 1:100 dilution.
Personal Communication. Elena Pugacheva, FoxChase Cancer Center, Philadelphia, PA.Immunoprecipitation
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Gene Info — NEDD9
Entrez GeneID
4739Protein Accession#
NP_006394;Q14511Gene Name
NEDD9
Gene Alias
CAS-L, CAS2, CASL, CASS2, HEF1, dJ49G10.2, dJ761I2.1
Gene Description
neural precursor cell expressed, developmentally down-regulated 9
Omim ID
602265Gene Ontology
HyperlinkGene Summary
O
Other Designations
Cas scaffolding protein family member 2|Crk-associated substrate related|OTTHUMP00000016030|cas-like docking|dJ49G10.2 (Enhancer of Filamentation 1 (HEF1))|dJ761I2.1 (enhancer of filamentation (HEF1))|enhancer of filamentation 1
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Interactome
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Disease
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Publication Reference
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Crk-associated substrate (Cas) family member, NEDD9, is regulated in human neuroblastoma cells and in the embryonic hindbrain by all-trans retinoic acid.
Merrill RA, See AW, Wertheim ML, Clagett-Dame M.
Developmental Dynamics 2004 Nov; 231(3):564.
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Regulation of HEF1 expression and phosphorylation by TGF-beta 1 and cell adhesion.
Zheng M, McKeown-Longo PJ.
The Journal of Biological Chemistry 2002 Oct; 277(42):39599.
Application：WB-Ce, Human, Fibroblasts.
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Human enhancer of filamentation 1, a novel p130cas-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae.
Law SF, Estojak J, Wang B, Mysliwiec T, Kruh G, Golemis EA.
Molecular and Cellular Biology 1996 Jul; 16(7):3327.
Application：IF, IP, Mouse, Human, 3Y1, HeLa, NIH/3T3 cells, Recombinant protein.
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Crk-associated substrate (Cas) family member, NEDD9, is regulated in human neuroblastoma cells and in the embryonic hindbrain by all-trans retinoic acid.
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