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Last updated: 2016/11/27
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NEDD9 monoclonal antibody, clone 14A11

  • Catalog # : MAB2091
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant NEDD9.
  • Immunogen:
  • Recombinant GST fusion protein corresponding to amino acids 82-398 of human NEDD9.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • This Protein A purified antibody is directed against human HEF1 protein. Reactivity against multiple isoforms is expected. Specificity was determined by partial epitope mapping.
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Western Blot (1:500)
    Immunofluorescence (1:100)
    Immunoprecipitation (1:100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blotting using NEDD9 monoclonal antibody, clone 14A11 (Cat # MAB2091) shows detection of NEDD9 present in MCF-7 cells induced to express NEDD9 by tetracycline removal (right lane).
    See Pugacheva et al for details.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence microscopyusing NEDD9 monoclonal antibody, clone 14A11 (Cat # MAB2091) shows detectionof NEDD9 localized at the centrosome (bright dots) and focal adhesion sites.
    The antibody was used at a 1:100 dilution.
    Personal Communication. Elena Pugacheva, FoxChase Cancer Center, Philadelphia, PA.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 4739
  • Protein Accession#:
  • NP_006394;Q14511
  • Gene Name:
  • NEDD9
  • Gene Alias:
  • CAS-L,CAS2,CASL,CASS2,HEF1,dJ49G10.2,dJ761I2.1
  • Gene Description:
  • neural precursor cell expressed, developmentally down-regulated 9
  • Other Designations:
  • Cas scaffolding protein family member 2,Crk-associated substrate related,OTTHUMP00000016030,cas-like docking,dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)),dJ761I2.1 (enhancer of filamentation (HEF1)),enhancer of filamentation 1
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