VCL monoclonal antibody, clone AIG-22
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) HepG2, (2) HeLa cell lysate.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human VCL.
Immunogen
A synthetic peptide corresponding to human VCL.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:50)
Immunocytochemistry (1:50-200)
Immunofluorescence (1:50-200)
Immunohistochemistry (1:50-200)
Western Blot (1:500-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) HepG2, (2) HeLa cell lysate.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — VCL
Entrez GeneID
7414Protein Accession#
P18206Gene Name
VCL
Gene Alias
CMD1W, MVCL
Gene Description
vinculin
Gene Ontology
HyperlinkGene Summary
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000019861|OTTHUMP00000043885|metavinculin
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Interactome
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Pathway
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Disease
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