VCAM1 monoclonal antibody, clone DCA-22
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human VCAM1.
Immunogen
A synthetic peptide corresponding to human VCAM1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunoprecipitation (1:20)
Immunocytochemistry (1:50-200)
Immunofluorescence (1:50-200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-200)
Western Blot (1:500-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot (Tissue lysate) analysis of mouse kidney lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human tonsil.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — VCAM1
Entrez GeneID
7412Protein Accession#
P19320Gene Name
VCAM1
Gene Alias
CD106, DKFZp779G2333, INCAM-100, MGC99561
Gene Description
vascular cell adhesion molecule 1
Omim ID
192225Gene Ontology
HyperlinkGene Summary
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
CD106 antigen|OTTHUMP00000012650|OTTHUMP00000012651
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Interactome
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