VAMP8 monoclonal antibody, clone AOBO-22
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human VAMP8.
Immunogen
A synthetic peptide corresponding to human VAMP8.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunoprecipitation (1:50)
Immunocytochemistry (1:50-200)
Immunofluorescence (1:50-200)
Immunohistochemistry (1:50-200)
Western Blot (1:500-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) HeLa, (2) NIH/3T3, (3) PC-12 cell lysate.Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — VAMP8
Entrez GeneID
8673Protein Accession#
Q9BV40Gene Name
VAMP8
Gene Alias
EDB
Gene Description
vesicle-associated membrane protein 8 (endobrevin)
Omim ID
603177Gene Ontology
HyperlinkGene Summary
Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF-attachment protein (alpha-SNAP), most likely through an VAMP8-containing SNARE complex. [provided by RefSeq
Other Designations
endobrevin|vesicle-associated membrane protein 8
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Interactome
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Pathway
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Disease
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