VAMP2 monoclonal antibody, clone AAEG-22
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human VAMP2.
Immunogen
A synthetic peptide corresponding to human VAMP2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunoprecipitation (1:50)
Immunocytochemistry (1:50-200)
Immunofluorescence (1:50-200)
Western Blot (1:1000-5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot (Tissue lysate) analysis of mouse brain lysate.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — VAMP2
Entrez GeneID
6844Protein Accession#
P63027Gene Name
VAMP2
Gene Alias
FLJ11460, SYB2, VAMP-2
Gene Description
vesicle-associated membrane protein 2 (synaptobrevin 2)
Omim ID
185881Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq
Other Designations
synaptobrevin 2|vesicle associated membrane protein 2
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