USP14 monoclonal antibody, clone ABGI-21
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa, Lane 2: RAW 2647 and Lane 3: C6 cell lysates with USP14 monoclonal antibody, clone ABGI-21 (Cat # MAB20834).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human USP14.
Immunogen
A synthetic peptide corresponding to human USP14.
Host
Rabbit
Theoretical MW (kDa)
56.069
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:60)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa, Lane 2: RAW 2647 and Lane 3: C6 cell lysates with USP14 monoclonal antibody, clone ABGI-21 (Cat # MAB20834).Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — USP14
Entrez GeneID
9097Protein Accession#
P54578Gene Name
USP14
Gene Alias
TGT
Gene Description
ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Omim ID
607274Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
deubiquitinating enzyme 14|tRNA-guanine transglycosylase, 60-kD subunit|ubiquitin carboxyl-terminal hydrolase 14|ubiquitin specific protease 14|ubiquitin specific protease 14 (tRNA-guanine transglycosylase)|ubiquitin thiolesterase 14|ubiquitin-specific pr
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Interactome
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