UBA1 monoclonal antibody, clone IAC-21
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell lysate with UBA1 monoclonal antibody, clone IAC-21 (Cat # MAB20826).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human UBA1.
Immunogen
A synthetic peptide corresponding to human UBA1.
Host
Rabbit
Theoretical MW (kDa)
117.849
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:20)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell lysate with UBA1 monoclonal antibody, clone IAC-21 (Cat # MAB20826).Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — UBA1
Entrez GeneID
7317Protein Accession#
P22314Gene Name
UBA1
Gene Alias
A1S9, A1S9T, A1ST, AMCX1, GXP1, MGC4781, SMAX2, UBA1A, UBE1, UBE1X
Gene Description
ubiquitin-like modifier activating enzyme 1
Omim ID
314370Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq
Other Designations
A1S9T and BN75 temperature sensitivity complementing|OTTHUMP00000023197|OTTHUMP00000023198|UBA1, ubiquitin-activating enzyme E1 homolog A|ubiquitin-activating enzyme E1
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Interactome
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Pathway
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Disease
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