TOP1 monoclonal antibody, clone FIG-20
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell lysate with TOP1 monoclonal antibody, clone FIG-20 (Cat # MAB20797).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human TOP1.
Immunogen
A synthetic peptide corresponding to human TOP1.
Host
Rabbit
Theoretical MW (kDa)
90.726
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell lysate with TOP1 monoclonal antibody, clone FIG-20 (Cat # MAB20797).Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — TOP1
Entrez GeneID
7150Protein Accession#
P11387Gene Name
TOP1
Gene Alias
TOPI
Gene Description
topoisomerase (DNA) I
Omim ID
126420Gene Ontology
HyperlinkGene Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq
Other Designations
DNA topoisomerase I|OTTHUMP00000031713|type I DNA topoisomerase
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Interactome
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Disease
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