TIA1 monoclonal antibody, clone AAHD-20
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: Jurkat and Lane 2: NIH/3T3 cell lysates with TIA1 monoclonal antibody, clone AAHD-20 (Cat # MAB20784).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human TIA1.
Immunogen
A synthetic peptide corresponding to human TIA1.
Host
Rabbit
Theoretical MW (kDa)
42.963
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: Jurkat and Lane 2: NIH/3T3 cell lysates with TIA1 monoclonal antibody, clone AAHD-20 (Cat # MAB20784).Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — TIA1
Entrez GeneID
7072Protein Accession#
P31483Gene Name
TIA1
Gene Alias
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Gene Description
TIA1 cytotoxic granule-associated RNA binding protein
Omim ID
603518Gene Ontology
HyperlinkGene Summary
The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature. [provided by RefSeq
Other Designations
T-cell-restricted intracellular antigen-1|TIA1 cytotoxic granule-associated RNA-binding protein|TIA1 protein|cytotoxic granule-associated RNA-binding protein|nucleolysin TIA-1 isoform p40|p40-TIA-1 (containing p15-TIA-1)
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Interactome
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+1-909-992-0619
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