RNF20 monoclonal antibody, clone FC-18
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: 293 and Lane 2: HeLa cell lysates with RNF20 monoclonal antibody, clone FC-18 (Cat # MAB20677).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human RNF20.
Immunogen
A synthetic peptide corresponding to human RNF20.
Host
Rabbit
Theoretical MW (kDa)
113.662
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: 293 and Lane 2: HeLa cell lysates with RNF20 monoclonal antibody, clone FC-18 (Cat # MAB20677).Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — RNF20
Entrez GeneID
56254Protein Accession#
Q5VTR2Gene Name
RNF20
Gene Alias
BRE1, BRE1A, FLJ11189, FLJ20382, KIAA2779, MGC129667, MGC129668, hBRE1
Gene Description
ring finger protein 20
Omim ID
607699Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3. [provided by RefSeq
Other Designations
BRE1 E3 ubiquitin ligase homolog|homolog of S. cerevisiae BRE1
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