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Last updated: 2022/5/15
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RPTOR monoclonal antibody, clone EGB-18 

  • Catalog # : MAB20661
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human RPTOR.
  • Immunogen:
  • A synthetic peptide corresponding to human RPTOR.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (1:50)
    Immunocytochemistry (1:50-1:100)
    Immunofluorescence (1:50-1:100)
    Immunohistochemistry (1:50-1:100)
    Immunoprecipitation (1:30)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of MCF-7 cell lysate using RPTOR monoclonal antibody, clone EGB-18.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Gene Information
  • Gene Name:
  • RPTOR
  • Gene Alias:
  • KOG1,Mip1
  • Gene Description:
  • regulatory associated protein of MTOR, complex 1
  • Gene Summary:
  • This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats,regulatory associated protein of mTOR
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