DACT3 monoclonal antibody, clone 2A5
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: mouse brain tissue lysate, Lane 2: 293T cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human gastric cancer tissue were incubated with DACT3 monoclonal antibody, clone 2A5 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant DACT3.
Immunogen
Recombinant protein corresponding to amino acids 89-202 of human DACT3.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: mouse brain tissue lysate, Lane 2: 293T cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human gastric cancer tissue were incubated with DACT3 monoclonal antibody, clone 2A5 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Enzyme-linked Immunoabsorbent Assay
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Gene Info — DACT3
Entrez GeneID
147906Protein Accession#
NP_659493Gene Name
DACT3
Gene Alias
MGC15476, RRR1
Gene Description
dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis)
Omim ID
611112Gene Ontology
HyperlinkGene Summary
antagonist of beta-catenin
Other Designations
arginine rich region 1|thymus expressed gene 3-like
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Interactome
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Publication Reference
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DACT3 is an epigenetic regulator of Wnt/beta-catenin signaling in colorectal cancer and is a therapeutic target of histone modifications.
Jiang X, Tan J, Li J, Kivimae S, Yang X, Zhuang L, Lee PL, Chan MT, Stanton LW, Liu ET, Cheyette BN, Yu Q.
Cancer Cell 2008 Jun; 13(6):529.
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Three Dact gene family members are expressed during embryonic development and in the adult brains of mice.
Fisher DA, Kivimäe S, Hoshino J, Suriben R, Martin PM, Baxter N, Cheyette BN.
Developmental Dynamics 2006 Sep; 235(9):2620.
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DACT3 is an epigenetic regulator of Wnt/beta-catenin signaling in colorectal cancer and is a therapeutic target of histone modifications.
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