PPP1CA monoclonal antibody, clone AFC-16
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of (1) Jurkat cell lysate, (2) HeLa cell lysate using PPP1CA monoclonal antibody, clone AFC-16.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human pancreas using PPP1CA monoclonal antibody, clone AFC-16.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human PPP1CA.
Immunogen
A synthetic peptide corresponding to human PPP1CA.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of (1) Jurkat cell lysate, (2) HeLa cell lysate using PPP1CA monoclonal antibody, clone AFC-16.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human pancreas using PPP1CA monoclonal antibody, clone AFC-16.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — PPP1CA
Entrez GeneID
5499Protein Accession#
P62136Gene Name
PPP1CA
Gene Alias
MGC15877, MGC1674, PP-1A, PPP1A
Gene Description
protein phosphatase 1, catalytic subunit, alpha isoform
Omim ID
176875Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
protein phosphatase 1, catalytic subunit, alpha|serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit
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Interactome
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Pathway
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