PLEC1 monoclonal antibody, clone AFE-16

Catalog # MAB20605

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Size:100 uL
Price: USD $ 428.00
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Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western Blot analysis of Jurkat cell lysate using PLEC1 monoclonal antibody, clone AFE-16.

  • Specifications

    Product Description

    Rabbit monoclonal antibody raised against synthetic peptide of human PLEC1.

    Immunogen

    A synthetic peptide corresponding to human PLEC1.

    Host

    Rabbit

    Reactivity

    Human

    Form

    Liquid

    Purification

    Affinity purification

    Isotype

    IgG

    Recommend Usage

    Immunohistochemistry (1:50-1:100)
    Immunoprecipitation (1:30)
    Western Blot (1:1000-1:2000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).

    Storage Instruction

    Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western Blot analysis of Jurkat cell lysate using PLEC1 monoclonal antibody, clone AFE-16.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunoprecipitation

  • Gene Info — PLEC1

    Entrez GeneID

    5339

    Protein Accession#

    Q15149

    Gene Name

    PLEC1

    Gene Alias

    EBS1, EBSO, HD1, PCN, PLEC1b, PLTN

    Gene Description

    plectin 1, intermediate filament binding protein 500kDa

    Omim ID

    131950 226670 601282

    Gene Ontology

    Hyperlink

    Gene Summary

    Plectin is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes (for reviews see PMID: 9701547, 11854008 and 17499243). Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 (PMID: 8633055, 8698233). The plectin gene locus in mouse on chromosome 15 has been analyzed in detail (PMID: 10556294, 14559777), revealing a genomic exon-intron organization with well over 40 exons spanning over 62 kb and an unusual 5' transcript complexity of plectin isoforms. Eleven exons (1-1j) have been identified that alternatively splice directly into a common exon 2 which is the first exon to encode plectin's highly conserved actin binding domain (ABD). Three additional exons (-1, 0a, and 0) splice into an alternative first coding exon (1c), and two additional exons (2alpha and 3alpha) are optionally spliced within the exons encoding the acting binding domain (exons 2-8). Analysis of the human locus has identified eight of the eleven alternative 5' exons found in mouse and rat (PMID: 14672974). Furthermore, isoforms lacking the central rod domain encoded by exon 31 have been detected in mouse and rat (PMID:10556294, 9177781), and as judged by molecular size, have also been detected in human on the protein level (PMID: 11441066, 10780662). It has been shown that the short alternative amino-terminal sequences encoded by the different first exons direct the targeting of the various isoforms to distinct subcellular locations (PMID: 14559777). As the expression of specific plectin isoforms was found to be dependent on cell type (tissue) and stage of development (PMID: 10556294, 12542521, 17389230) it appears that each cell type (tissue) contains a unique set (proportion and composition) of plectin isoforms, as if custom-made for specific requirements of the particular cells. Concordantly, individual isoforms were found to carry out distinct and specific functions (PMID: 14559777, 12542521, 18541706). In 1996 a number of groups reported that patients

    Other Designations

    epidermolysis bullosa simplex 1 (Ogna)|hemidesmosomal protein 1|plectin 1|plectin 1, intermediate filament binding protein, 500kD

  • Interactomes
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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