Cell lysates of A-549 (lane 1), 293T (lane 2) and mouse kidney (lane 3) (35 ug) were resolved by SDS-PAGE and probed with CHGA monoclonal antibody, clone 3A5 (Cat # MAB2060) (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq
betagranin (N-terminal fragment of chromogranin A),chromogranin A,parathyroid secretory protein 1