SMAD5 (phospho S463/S465) monoclonal antibody, clone HAF-19
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human SMAD5.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding of human SMAD5.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of (1) HeLa cell lysate, (2) HeLa cell treated with BMP-4 lysate using SMAD5 (phospho S463/S465) monoclonal antibody, clone HAF-19.Immunocytochemistry
Immunofluorescence
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Gene Info — SMAD5
Entrez GeneID
4090Protein Accession#
Q99717Gene Name
SMAD5
Gene Alias
DKFZp781C1895, DKFZp781O1323, Dwfc, JV5-1, MADH5
Gene Description
SMAD family member 5
Omim ID
603110Gene Ontology
HyperlinkGene Summary
mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD
Other Designations
MAD homolog 5|MAD, mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD, mothers against DPP homolog 5|mothers against decapentaplegic homolog 5|mothers against decapentaplegic, drosophila, homolog of, 5
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Interactome
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Pathway
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Disease
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