SIRT1 (phospho T530) monoclonal antibody, clone HAI-19

Product Description:
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human SIRT1.

Immunogen:
A synthetic phosphopeptide corresponding to residues surrounding T530 of human SIRT1.

Recommend Usage:
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.

Storage Buffer:
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).

Storage Instruction:
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Western Blot (Cell lysate)
Western Blot analysis of (1) 293T cell lysate, (2) 293T cell treated with Calyculin A using SIRT1 (phospho T530) monoclonal antibody, clone HAI-19.

Gene Summary:
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq