Tollip is a mediator of protein sumoylation. Ciarrocchi A, D'Angelo R, Cordiglieri C, Rispoli A, Santi S, Riccio M, Carone S, Mancia AL, Paci S, Cipollini E, Ambrosetti D, Melli M.PLoS One. 2009;4(2):e4404. Epub 2009 Feb 9.
Cell lysate of Jurkat (30 ug) was resolved by SDS-PAGE and probed with TOLLIP monoclonal antibody, clone k1B17 (Cat # MAB2057) (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.