TOLLIP monoclonal antibody, clone k1B17
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate, Lane 2: HepG2 cell lysate, Lane 3: HeLa cell lysate.
Immunofluorescence
Immunofluorescence analysis of THP-1 cells. The cell was stained with TOLLIP monoclonal antibody, clone k1B17 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant TOLLIP.
Immunogen
Recombinant protein corresponding to amino acids 61-274 of human TOLLIP.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate, Lane 2: HepG2 cell lysate, Lane 3: HeLa cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of THP-1 cells. The cell was stained with TOLLIP monoclonal antibody, clone k1B17 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — TOLLIP
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Interactome
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Pathway
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Disease
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Publication Reference
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Tollip is a mediator of protein sumoylation.
Ciarrocchi A, D'Angelo R, Cordiglieri C, Rispoli A, Santi S, Riccio M, Carone S, Mancia AL, Paci S, Cipollini E, Ambrosetti D, Melli M.
PLoS One 2009 Feb; 4(2):e4404.
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Tollip regulates proinflammatory responses to interleukin-1 and lipopolysaccharide.
Didierlaurent A, Brissoni B, Velin D, Aebi N, Tardivel A, Kaslin E, Sirard JC, Angelov G, Tschopp J, Burns K.
Molecular and Cellular Biology 2006 Feb; 26(3):735.
Application：WB-Tr, Human, Mouse, HEK 293T cells, MEFs.
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Bench-to-bedside review: Toll-like receptors and their role in septic shock.
Opal SM, Huber CE.
Critical Care 2002 Apr; 6(2):125.
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Tollip is a mediator of protein sumoylation.
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