TREM2 monoclonal antibody, clone 2B5
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: MCF7 cell lysate, Lane 3: LNCaP cell lysate, Lane 4: mouse brain tissue lysate.
Immunofluorescence
Immunofluorescence analysis of Raw264.7 cells. The cell was stained with TREM2 monoclonal antibody, clone 2B5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant TREM2.
Immunogen
Recombinant protein corresponding to amino acids 19-161 of human TREM2.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG3, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: MCF7 cell lysate, Lane 3: LNCaP cell lysate, Lane 4: mouse brain tissue lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of Raw264.7 cells. The cell was stained with TREM2 monoclonal antibody, clone 2B5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. -
Gene Info — TREM2
Entrez GeneID
54209Protein Accession#
NP_061838Gene Name
TREM2
Gene Alias
TREM-2, Trem2a, Trem2b, Trem2c
Gene Description
triggering receptor expressed on myeloid cells 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a membrane protein that forms a receptor signaling complex with TYROBP. The encoded protein may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). [provided by RefSeq
Other Designations
OTTHUMP00000016368|OTTHUMP00000039771|triggering receptor expressed on monocytes 2|triggering receptor expressed on myeloid cells 2a
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Interactome
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Disease
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Publication Reference
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Microglial VPS35 deficiency impairs Aβ phagocytosis and Aβ-induced disease-associated microglia, and enhances Aβ associated pathology.
Xiao Ren, Lingling Yao, YongGang Wang, Lin Mei, Wen-Cheng Xiong.
Journal of Neuroinflammation 2022 Mar; 19(1):61.
Application：IF, Mouse, Mouse brain.
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Engagement of TREM2 by a novel monoclonal antibody induces activation of microglia and improves cognitive function in Alzheimer's disease models.
Michael Fassler, Maya Saban Rappaport, Clara Benaim Cuño, Jacob George.
Journal of Neuroinflammation 2021 Jan; 18(1):19.
Application：Dot-Tr, Human, HEK 293T cells.
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Microglial VPS35 deficiency impairs Aβ phagocytosis and Aβ-induced disease-associated microglia, and enhances Aβ associated pathology.
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