CDC42 (phospho S71) monoclonal antibody, clone IBB-3

Product Description:
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human CDC42.

Immunogen:
A synthetic phosphopeptide corresponding to residues surrounding S71 of human CDC42.

Recommend Usage:
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.

Storage Buffer:
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).

Storage Instruction:
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Western Blot (Cell lysate)
Western Blot analysis of (1) A431 cell lysate treated with LP, (2) A431 cell lysate using CDC42 (phospho S71) monoclonal antibody, clone IBB-3.

Gene Summary:
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq

Other Designations:
GTP-binding protein, 25kD,OTTHUMP00000002834,OTTHUMP00000002926,cell division cycle 42,cell division cycle 42 (GTP binding protein, 25kD),cell division cycle 42 (GTP-binding protein, 25kD),dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)),d