Rabbit monoclonal antibody raised against synthetic phosphopeptide of human PRKAR2A.
Immunogen:
A synthetic phosphopeptide corresponding to residues surrounding S99 of human PRKAR2A.
Host:
Rabbit
Reactivity:
Human
Form:
Liquid
Purification:
Affinity purification
Isotype:
IgG
Recommend Usage:
Immunocytochemistry (1:50-1:200) Immunofluorescence (1:50-1:200) Immunohistochemistry (1:50-1:200) Immunoprecipitation (1:50) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction:
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq
Other Designations:
cAMP-dependent protein kinase regulatory subunit RII alpha,cAMP-dependent protein kinase, regulatory subunit alpha 2,protein kinase A, RII-alpha subunit