PRKAR2A (phospho S99) monoclonal antibody, clone AOG-16
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More Files
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Western Blot (Cell lysate)
Western Blot analysis of K-562 cell lysate using PRKAR2A (phospho S99) monoclonal antibody, clone AOG-16.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human PRKAR2A.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding S99 of human PRKAR2A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of K-562 cell lysate using PRKAR2A (phospho S99) monoclonal antibody, clone AOG-16.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — PRKAR2A
Entrez GeneID
5576Protein Accession#
P13861Gene Name
PRKAR2A
Gene Alias
MGC3606, PKR2, PRKAR2
Gene Description
protein kinase, cAMP-dependent, regulatory, type II, alpha
Omim ID
176910Gene Ontology
HyperlinkGene Summary
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq
Other Designations
cAMP-dependent protein kinase regulatory subunit RII alpha|cAMP-dependent protein kinase, regulatory subunit alpha 2|protein kinase A, RII-alpha subunit
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Interactome
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