JUND (phospho S255) monoclonal antibody, clone HFC-10
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of (1) HeLa cell lysate treated with Alkaline Phosphatase, (2) HeLa cell lysate using JUND (phospho S255) monoclonal antibody, clone HFC-10.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human JUND.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding S255 of human JUND.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:50)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of (1) HeLa cell lysate treated with Alkaline Phosphatase, (2) HeLa cell lysate using JUND (phospho S255) monoclonal antibody, clone HFC-10.Immunoprecipitation
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Gene Info — JUND
Entrez GeneID
3727Protein Accession#
P17535Gene Name
JUND
Gene Alias
AP-1
Gene Description
jun D proto-oncogene
Omim ID
165162Gene Ontology
HyperlinkGene Summary
The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. It has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternate translation initiation site usage results in the production of different isoforms. [provided by RefSeq
Other Designations
JunD-FL isoform|activator protein 1|transcription factor jun-D
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Interactome
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Pathway
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Disease
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