AVEN monoclonal antibody, clone 3G4
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse kidney.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human breast lobule tissue were incubated with AVEN monoclonal antibody, clone 3G4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant AVEN.
Immunogen
Recombinant protein corresponding to amino acids 254-362 of human AVEN.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse kidney.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human breast lobule tissue were incubated with AVEN monoclonal antibody, clone 3G4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Enzyme-linked Immunoabsorbent Assay
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Gene Info — AVEN
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Interactome
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Disease
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Publication Reference
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Aven and Bcl-xL enhance protection against apoptosis for mammalian cells exposed to various culture conditions.
Figueroa B Jr, Chen S, Oyler GA, Hardwick JM, Betenbaugh MJ.
Biotechnology and Bioengineering 2004 Mar; 85(6):589.
Application：WB, Mouse, CHO cells.
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Aven, a novel inhibitor of caspase activation, binds Bcl-xL and Apaf-1.
Chau BN, Cheng EH, Kerr DA, Hardwick JM.
Molecular Cell 2000 Jul; 6(1):31.
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Aven and Bcl-xL enhance protection against apoptosis for mammalian cells exposed to various culture conditions.
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