Rabbit monoclonal antibody raised against synthetic phosphopeptide of yeast HTB1.
Immunogen:
A synthetic phosphopeptide corresponding to residues surrounding T129 of yeast HTB1.
Host:
Rabbit
Reactivity:
Yeast
Form:
Liquid
Purification:
Affinity purification
Isotype:
IgG
Recommend Usage:
Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction:
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot analysis of Saccharomyces cerevisiae cell lysate treated with Methyl methanesulfonate using HTB1 (phospho T129) monoclonal antibody, clone DEI-8.
One of two nearly identical (see HTB2) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation