HIST1H1E (phospho T17) monoclonal antibody, clone DGH-8
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell lysate using HIST1H1E (phospho T17) monoclonal antibody, clone DGH-8.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded mouse colon using HIST1H1E (phospho T17) monoclonal antibody, clone DGH-8.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human HIST1H1E.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding T17 of human HIST1H1E.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell lysate using HIST1H1E (phospho T17) monoclonal antibody, clone DGH-8.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded mouse colon using HIST1H1E (phospho T17) monoclonal antibody, clone DGH-8.Immunocytochemistry
Immunofluorescence
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Gene Info — HIST1H1E
Entrez GeneID
3008Protein Accession#
P10412Gene Name
HIST1H1E
Gene Alias
H1.4, H1F4, MGC116819, dJ221C16.5
Gene Description
histone cluster 1, H1e
Omim ID
142220Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations
H1 histone family, member 4|histone 1, H1e|histone H1b
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