EPM2A monoclonal antibody, clone k2A3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with EPM2A monoclonal antibody, clone k2A3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant EPM2A.
Immunogen
Recombinant protein corresponding to amino acids 243-331 of human EPM2A.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with EPM2A monoclonal antibody, clone k2A3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPM2A
Entrez GeneID
7957Protein Accession#
NP_005661Gene Name
EPM2A
Gene Alias
EPM2, MELF
Gene Description
epilepsy, progressive myoclonus type 2A, Lafora disease (laforin)
Gene Ontology
HyperlinkGene Summary
This gene encodes a dual-specificity phosphatase that associates with polyribosomes. The encoded protein may be involved in the regulation of glycogen metabolism. Mutations in this gene have been associated with myoclonic epilepsy of Lafora. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000017360|epilepsy, progressive myoclonus type 2, Lafora disease (laforin)|laforin
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Interactome
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Disease
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Publication Reference
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Laforin is a glycogen phosphatase, deficiency of which leads to elevated phosphorylation of glycogen in vivo.
Tagliabracci VS, Turnbull J, Wang W, Girard JM, Zhao X, Skurat AV, Delgado-Escueta AV, Minassian BA, Depaoli-Roach AA, Roach PJ.
PNAS 2007 Nov; 104(49):19262.
Application：WB, Mouse, Mouse brain.
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Relationship between glycogen accumulation and the laforin dual specificity phosphatase.
Wang W, Parker GE, Skurat AV, Raben N, DePaoli-Roach AA, Roach PJ.
Biochemical and Biophysical Research Communications 2006 Nov; 350(3):588.
Application：WB-Ti, Mouse, Mouse skeletal muscle.
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Genotype-phenotype correlations for EPM2A mutations in Lafora's progressive myoclonus epilepsy: exon 1 mutations associate with an early-onset cognitive deficit subphenotype.
Ganesh S, Delgado-Escueta AV, Suzuki T, Francheschetti S, Riggio C, Avanzini G, Rabinowicz A, Bohlega S, Bailey J, Alonso ME, Rasmussen A, Thomson AE, Ochoa A, Prado AJ, Medina MT, Yamakawa K.
Human Molecular Genetics 2002 May; 11(11):1263.
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Laforin is a glycogen phosphatase, deficiency of which leads to elevated phosphorylation of glycogen in vivo.
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