EIF2S1 (phospho S51) monoclonal antibody, clone IO-5
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More Files
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human EIF2S1.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding S51 of human EIF2S1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:40)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of (1)HeLa cell lysates treated with Calyculin A,(2) Untreated HeLa cell lysates using EIF2S1 (phospho S51) monoclonal antibody, clone IO-5.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human colon cancer using EIF2S1 (phospho S51) monoclonal antibody, clone IO-5.Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — EIF2S1
Entrez GeneID
1965Protein Accession#
P05198Gene Name
EIF2S1
Gene Alias
EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A
Gene Description
eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Omim ID
603907Gene Ontology
HyperlinkGene Summary
The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM
Other Designations
eIF-2-alpha|eukaryotic translation initiation factor 2, subunit 1 (alpha, 35kD )
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