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Last updated: 2017/3/26
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STEAP1 monoclonal antibody, clone J2D2

  • Catalog # : MAB2051
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant STEAP1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-70 of human STEAP1.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Cell lysates of LNCap (Lane 1, 30 ug) and rat testis (Lane 2, 30 ug) were resolved by SDS-PAGE and probed with STEAP1 monoclonal antibody, clone J2D2 (Cat # MAB2051) (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Paraffin embedded sections of human breast cancer (A) and prostate hyperplasia tissue (B) were incubated with STEAP1 monoclonal antibody, clone J2D2 (Cat # MAB2051) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of human LNCaP cell colony with STEAP1 monoclonal antibody, clone J2D2 (Cat # MAB2051).
  • ELISA
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • STEAP1
  • Gene Alias:
  • MGC19484,PRSS24,STEAP
  • Gene Description:
  • six transmembrane epithelial antigen of the prostate 1
  • Gene Summary:
  • This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions. [provided by RefSeq
  • Other Designations:
  • six transmembrane epithelial antigen of the prostate
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