UBE2L6 monoclonal antibody, clone k1H3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of MCF7 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human breast lobule tissue were incubated with UBE2L6 monoclonal antibody, clone k1H3 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with UBE2L6 monoclonal antibody, clone k1H3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant UBE2L6.
Immunogen
Recombinant protein corresponding to amino acids 1-152 of human UBE2L6.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MCF7 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human breast lobule tissue were incubated with UBE2L6 monoclonal antibody, clone k1H3 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with UBE2L6 monoclonal antibody, clone k1H3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — UBE2L6
Entrez GeneID
9246Protein Accession#
NP_004214Gene Name
UBE2L6
Gene Alias
MGC40331, RIG-B, UBCH8
Gene Description
ubiquitin-conjugating enzyme E2L 6
Omim ID
603890Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
retinoic acid induced gene B protein|ubiquitin carrier protein|ubiquitin-protein ligase
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Interactome
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Pathway
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Publication Reference
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Genomic organization of the human ubiquitin-conjugating enzyme gene, UBE2L6 on chromosome 11q12.
Ardley HC, Rose SA, Tan N, Leek JP, Markham AF, Robinson PA.
Cytogenetics and Cell Genetics 2000 Feb; 89(1-2):137.
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The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1.
Moynihan TP, Ardley HC, Nuber U, Rose SA, Jones PF, Markham AF, Scheffner M, Robinson PA.
The Journal of Biological Chemistry 1999 Oct; 274(43):30963.
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Physical interaction between specific E2 and Hect E3 enzymes determines functional cooperativity.
Kumar S, Kao WH, Howley PM.
The Journal of Biological Chemistry 1997 May; 272(21):13548.
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Genomic organization of the human ubiquitin-conjugating enzyme gene, UBE2L6 on chromosome 11q12.
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