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Last updated: 2021/8/8

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Methyl-Histone H3 (dimethyl K4) monoclonal antibody, clone AFF-8 

  • Catalog # : MAB20487
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic dimethyl peptide of Methyl-Histone H3 (dimethyl K4).
  • Immunogen:
  • A synthetic dimethyl peptide corresponding to residues surrounding K4 of Methyl-Histone H3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ChIP
    Flow Cytometry (1:50)
    Immunocytochemistry (1:50-1:200)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
    Immunoprecipitation (1:50)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of HeLa cell lysate with Methyl-Histone H3 (dimethyl K4) monoclonal antibody, clone AFF-8 (Cat # MAB20487).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver with Methyl-Histone H3 (dimethyl K4) monoclonal antibody, clone AFF-8 (Cat # MAB20487).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Application Image
  • ChIP
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
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