MAP2K5 monoclonal antibody, clone HCE-13
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More Files
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Western Blot (Cell lysate)
Western Blot analysis of HeLa cell lysate with MAP2K5 monoclonal antibody, clone HCE-13 (Cat # MAB20481).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human MAP2K5.
Immunogen
A synthetic peptide corresponding to human MAP2K5.
Host
Rabbit
Theoretical MW (kDa)
50.112
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HeLa cell lysate with MAP2K5 monoclonal antibody, clone HCE-13 (Cat # MAB20481).Immunocytochemistry
Immunofluorescence
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Gene Info — MAP2K5
Entrez GeneID
5607Protein Accession#
Q13163Gene Name
MAP2K5
Gene Alias
HsT17454, MAPKK5, MEK5, PRKMK5
Gene Description
mitogen-activated protein kinase kinase 5
Omim ID
602520Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
MAP kinase kinase MEK5b|MAPK/ERK kinase 5|dual specificity mitogen-activated protein kinase kinase 5
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Interactome
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Pathway
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Disease
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