Western blot analysis of Lane 1: CTSB recombinant protein, Lane 2: CTSD recombinant protein, Lane 3: CTSE recombinant protein, Lane 4: CTSF recombinant protein, Lane 5: CTSH recombinant protein, Lane 6: CTSK recombinant protein, Lane 7: CTSL recombinant protein, Lane 8: CTSS recombinant protein, Lane 9: CTSW recombinant protein, Lane 10: CTSZ recombinant protein.
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of A549 cells. The cell was stained with CTSL1 monoclonal antibody, clone AT18F6 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of A549 cells. The cell was stained with CTSL1 monoclonal antibody, clone AT18F6 at 2-5 ug for 1x1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations:
OTTHUMP00000021601,OTTHUMP00000021602,OTTHUMP00000063566,cathepsin L,major excreted protein