CTSL1 monoclonal antibody, clone AT18F6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A-549 cell lysate.
Western Blot (Recombinant protein)
Western blot analysis of Lane 1: CTSB recombinant protein, Lane 2: CTSD recombinant protein, Lane 3: CTSE recombinant protein, Lane 4: CTSF recombinant protein, Lane 5: CTSH recombinant protein, Lane 6: CTSK recombinant protein, Lane 7: CTSL recombinant protein, Lane 8: CTSS recombinant protein, Lane 9: CTSW recombinant protein, Lane 10: CTSZ recombinant protein.
Immunofluorescence
Immunofluorescence analysis of A549 cells. The cell was stained with CTSL1 monoclonal antibody, clone AT18F6 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of A549 cells. The cell was stained with CTSL1 monoclonal antibody, clone AT18F6 at 2-5 ug for 1x1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CTSL1.
Immunogen
Recombinant protein corresponding to amino acids 18-333 of human CTSL1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A-549 cell lysate.Western Blot (Recombinant protein)
Western blot analysis of Lane 1: CTSB recombinant protein, Lane 2: CTSD recombinant protein, Lane 3: CTSE recombinant protein, Lane 4: CTSF recombinant protein, Lane 5: CTSH recombinant protein, Lane 6: CTSK recombinant protein, Lane 7: CTSL recombinant protein, Lane 8: CTSS recombinant protein, Lane 9: CTSW recombinant protein, Lane 10: CTSZ recombinant protein.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of A549 cells. The cell was stained with CTSL1 monoclonal antibody, clone AT18F6 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of A549 cells. The cell was stained with CTSL1 monoclonal antibody, clone AT18F6 at 2-5 ug for 1x1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). -
Gene Info — CTSL1
Entrez GeneID
1514Protein Accession#
NP_001903Gene Name
CTSL1
Gene Alias
CATL, CTSL, FLJ31037, MEP
Gene Description
cathepsin L1
Omim ID
116880Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000021601|OTTHUMP00000021602|OTTHUMP00000063566|cathepsin L|major excreted protein
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Interactome
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Pathway
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Publication Reference
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Cathepsin L inhibition suppresses drug resistance in vitro and in vivo: a putative mechanism.
Zheng X, Chu F, Chou PM, Gallati C, Dier U, Mirkin BL, Mousa SA, Rebbaa A.
American Journal of Physiology. Cell Physiology 2009 Jan; 296(1):C65.
Application：WB-Ce, Human, SaOS2 cells.
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The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling.
Reinheckel T, Hagemann S, Dollwet-Mack S, Martinez E, Lohmuller T, Zlatkovic G, Tobin DJ, Maas-Szabowski N, Peters C.
Journal of Cell Science 2005 Aug; 118(Pt 15):3387.
Application：IHC-P, Mouse, Mouse skin.
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Processing and activation of lysosomal proteinases.
Ishidoh K, Kominami E.
Biological Chemistry 2002 Dec; 383(12):1827.
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Cathepsin L inhibition suppresses drug resistance in vitro and in vivo: a putative mechanism.
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