Streptomyces avidinii Streptavidin monoclonal antibody, clone 1C2
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Streptomyces avidinii Streptavidin.
Immunogen
Recombinant protein corresponding to amino acids 25-183 of Streptomyces avidinii Streptavidin.
Host
Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Recombinant protein)
Western blot analysis of recombinant Streptavidin protein (17 kDa) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Streptavidin (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Enzyme-linked Immunoabsorbent Assay
-
Publication Reference
-
A monovalent streptavidin with a single femtomolar biotin binding site.
Howarth M, Chinnapen DJ, Gerrow K, Dorrestein PC, Grandy MR, Kelleher NL, El-Husseini A, Ting AY.
Nature Methods 2006 Apr; 3(4):267.
-
The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures.
Holmberg A, Blomstergren A, Nord O, Lukacs M, Lundeberg J, Uhlen M.
Electrophoresis 2005 Feb; 26(3):501.
-
A monovalent streptavidin with a single femtomolar biotin binding site.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com