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Last updated: 2021/1/10
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FADD monoclonal antibody, clone J1D2 

  • Catalog # : MAB2044
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant FADD.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-208 of human FADD.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2b, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA
    Immunocytochemistry
    Immunofluorescence
    Immunohistochemistry
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: Raw264.7 cell lysate, Lane 3: MCF7 cell lysate, Lane 4: A431 cell lysate, Lane 5: Ramos cell lysate, Lane 6: Raji cell lysate, Lane 7: Balb/3T3 cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of human breast cancer tissue were incubated with FADD monoclonal antibody, clone J1D2 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells. The cell was stained with FADD monoclonal antibody, clone J1D2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 8772
  • Gene Name:
  • FADD
  • Gene Alias:
  • GIG3,MGC8528,MORT1
  • Gene Description:
  • Fas (TNFRSF6)-associated via death domain
  • Gene Summary:
  • The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq
  • Other Designations:
  • Fas-associated via death domain,Fas-associating death domain-containing protein,Fas-associating protein with death domain,growth-inhibiting gene 3 protein,mediator of receptor-induced toxicity
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  • Interactome
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