Immunohistochemistry of of human seminoma tissue were incubated with NANOG monoclonal antibody, clone 5A10 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.