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Last updated: 2021/1/17
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NANOG monoclonal antibody, clone 5A10 

  • Catalog # : MAB2042
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant NANOG.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-154 of human NANOG.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2a, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunohistochemistry
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of NIH/3T3 cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of of human seminoma tissue were incubated with NANOG monoclonal antibody, clone 5A10 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Gene Name:
  • NANOG
  • Gene Alias:
  • -
  • Gene Description:
  • Nanog homeobox
  • Other Designations:
  • homeobox transcription factor Nanog,homeobox transcription factor Nanog-delta 48
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