IRF1 monoclonal antibody, clone DHE-9
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More Files
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human IRF1.
Immunogen
A synthetic peptide corresponding to human IRF1.
Host
Rabbit
Theoretical MW (kDa)
36.502
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
Immunoprecipitation (1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell lysate with IRF1 monoclonal antibody, clone DHE-9 (Cat # MAB20399).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of rat kidney with IRF1 monoclonal antibody, clone DHE-9 (Cat # MAB20399).Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — IRF1
Entrez GeneID
3659Protein Accession#
P10914Gene Name
IRF1
Gene Alias
IRF-1, MAR
Gene Description
interferon regulatory factor 1
Gene Ontology
HyperlinkGene Summary
IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion. [provided by RefSeq
Other Designations
interferon regulatory factor-1
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Interactome
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Disease
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