PPP1R14A monoclonal antibody, clone 4H10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A549 cell lysate, Lane 2. : NIH-3T3 cell lysate.
Immunofluorescence
Immunofluorescence analysis of A-549 cells. The cell was stained with PPP1R14A monoclonal antibody, clone 4H10 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PPP1R14A.
Immunogen
Recombinant protein corresponding to amino acids 1-147 of human PPP1R14A.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A549 cell lysate, Lane 2. : NIH-3T3 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of A-549 cells. The cell was stained with PPP1R14A monoclonal antibody, clone 4H10 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPP1R14A
Entrez GeneID
94274Protein Accession#
NP_150281Gene Name
PPP1R14A
Gene Alias
CPI-17, CPI17, PPP1INL
Gene Description
protein phosphatase 1, regulatory (inhibitor) subunit 14A
Omim ID
608153Gene Ontology
HyperlinkGene Summary
PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase (see MIM 603768). Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration.[supplied by OMIM
Other Designations
17-KDa protein|17-kDa PKC-potentiated inhibitory protein of PP1|PKC-potentiated inhibitory protein of PP1
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Interactome
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Pathway
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Publication Reference
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Up-regulation of myometrial RHO effector proteins (PKN1 and DIAPH1) and CPI-17 (PPP1R14A) phosphorylation in human pregnancy is associated with increased GTP-RHOA in spontaneous preterm labor.
Lartey J, Smith M, Pawade J, Strachan B, Mellor H, Lopez Bernal A.
Biology of Reproduction 2007 Jun; 76(6):971.
Application：WB-Ce, Human, myometrial cells.
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Phosphorylation of CPI-17, an inhibitor of myosin phosphatase, by protein kinase N.
Hamaguchi T, Ito M, Feng J, Seko T, Koyama M, Machida H, Takase K, Amano M, Kaibuchi K, Hartshorne DJ, Nakano T.
Biochemical and Biophysical Research Communications 2000 Aug; 274(3):825.
Application：WB-Re, Recombinant protein.
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Molecular cloning of a novel phosphorylation-dependent inhibitory protein of protein phosphatase-1 (CPI17) in smooth muscle: its specific localization in smooth muscle.
Eto M, Senba S, Morita F, Yazawa M.
FEBS Letters 1997 Jun; 410(2-3):356.
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Up-regulation of myometrial RHO effector proteins (PKN1 and DIAPH1) and CPI-17 (PPP1R14A) phosphorylation in human pregnancy is associated with increased GTP-RHOA in spontaneous preterm labor.
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