Western blot analysis of Lane 1: A549 cell lysate, Lane 2. : NIH-3T3 cell lysate.
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of A-549 cells. The cell was stained with PPP1R14A monoclonal antibody, clone 4H10 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase (see MIM 603768). Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration.[supplied by OMIM
Other Designations:
17-KDa protein,17-kDa PKC-potentiated inhibitory protein of PP1,PKC-potentiated inhibitory protein of PP1