The cell lysate of A-549 (30 ug) was resolved by SDS-PAGE and probed with PPP1R14A monoclonal antibody, clone 4H10 (Cat # MAB2039) (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase (see MIM 603768). Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration.[supplied by OMIM
17-KDa protein,17-kDa PKC-potentiated inhibitory protein of PP1,PKC-potentiated inhibitory protein of PP1