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Last updated: 2020/8/2
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HSPH1 monoclonal antibody, clone J1G12 

  • Catalog # : MAB2035
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant HSPH1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-858 of human HSPH1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2b, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: HepG2 cell lysate, Lane 3: NIH-3T3 cell lysate, Lane 4: MCF7 cell lysate, Lane 5: U-87 MG cell lysate, Lane 6: A549 cell lysate.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of Hep3B cells. The cell was stained with HSPH1 monoclonal antibody, clone J1G12 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow cytometry analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Gene Information
  • Gene Name:
  • HSPH1
  • Gene Alias:
  • DKFZp686M05240,HSP105,HSP105A,HSP105B,KIAA0201,NY-CO-25
  • Gene Description:
  • heat shock 105kDa/110kDa protein 1
  • Other Designations:
  • OTTHUMP00000018208,heat shock 105kD,heat shock 105kD alpha,heat shock 105kD beta,heat shock 105kDa protein 1
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