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Last updated: 2021/8/8

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HSPA1A monoclonal antibody, clone DGE-8 

  • Catalog # : MAB20340
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human HSPA1A.
  • Immunogen:
  • A synthetic peptide corresponding to human HSPA1A.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 70.052
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (1:100)
    Immunocytochemistry (1:50-1:100)
    Immunofluorescence (1:50-1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:500)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: K562 and Lane 2: 293T cell lysates with HSPA1A monoclonal antibody, clone DGE-8 (Cat # MAB20340).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast with HSPA1A monoclonal antibody, clone DGE-8 (Cat # MAB20340).
  • Immunocytochemistry
  • Immunofluorescence
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunofluorescence
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 3303
  • Gene Name:
  • HSPA1A
  • Gene Alias:
  • FLJ54303,FLJ54370,FLJ54392,FLJ54408,FLJ75127,HSP70-1,HSP70-1A,HSP70I,HSP72,HSPA1,HSPA1B
  • Gene Description:
  • heat shock 70kDa protein 1A
  • Gene Summary:
  • This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000029287,dnaK-type molecular chaperone HSP70-1,heat shock 70kD protein 1A,heat shock 70kDa protein 1B,heat shock-induced protein
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